Fig. 4.

mPR activity in ovarian cancer cells increases BAX gene expression in a JNK1/2-dependent manner. a SKOV-3 ovarian cancer cells treated for 24 h with progesterone (50 nM and 100 nM) demonstrated a significant increase in BAX gene expression versus vehicle treated cells but not when compared against each other as determined by quantitative PCR (qPCR) relative to the GAPDH housekeeping gene (mean ± SD, n=4; NS, not significant; asterisk, p≤ 0.05). b The JNK1/2 inhibitor SP 600125 (10 µ M), but not the p38 inhibitor SB 203580 (2.6 µ M), significantly decreased BAX gene expression in SKOV-3 ovarian cancer cells stimulated with progesterone (100 nM) for 24 h as determined by qPCR relative to the β-ACTIN housekeeping gene (mean ± SD, n=3; asterisk, p≤0.05)