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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Mol Oncol. 2013 Oct 28;8(1):142–149. doi: 10.1016/j.molonc.2013.10.007

Figure 5.

Figure 5

Cells with higher percentage of ALDH-1 more readily for tumor sphere and are resistant to 5-FU. A. From JHESO, ALDH-1+ and ALDH-1− cells are sorted by fluorescence activated cell sorting in 3 cell lines (FLO-1, JHESO, and OAPC) according to ALDEFLUOR detection kit following the protocol and tumor sphere assays were carried out in triplicate in ultra-low attachment plate in the tumor sphere medium. After 8-10 days in culture, the number and size of the tumor spheres were counted under a microscope. The ALDH-1+ cells formed larger tumor spheres and these are numerically shown in the bar graph below. B. The proliferation rate of ALDH1+ and ALDH-1− cells using the MTS proliferation assay on day 2 and day 6 demonstrate higher proliferative activity of ALDH-1+ cells. C. ALDH-1+ cells are more resistant to 5-FU at different concentrations.