Table 1. Virus RNA shedding determined by qRT-PCR in the feces of GIII.2 BoNoV (CV186-OH)-infected calves during the early stages of infection.

Calf #	Calf age when inoculated (day)	Inoculum titer (log10 GE/calf)	Viral titers (log10 GE/ml of rectal swab fluid or fecal sample)							PID when virus titer peaked
			Post-inoculation day (PID)
			0	1	2	3	4	5	6
1	6	11.9	<3.7a	<3.7	9.9b	10.6	EUc			≥ 3 (NDd)
2	5	11.9	<3.7	10.1	9.7	EU				1
3e	4	11.3	<3.7	11.9	11.9	10.9	11.2	10.9	10.4	1 and 2
4e	4	11.0	<3.7	11.6	10.3	10.1	10.1	9.6	8.9	1
5	4	11.0	<3.7	11.4 (EU)						≥ 1 (ND)
6	7	10.0	<3.7	10.7	10.1	10.4	8.7	8.8	EU	1
7	7	10.1	<3.7	<3.7	10.4	10.7	10.9	EU		≥ 4 (ND)

^aReal-time PCR-negative; <3.7 log₁₀ GE/ml (detection limit of the qRT-PCR for rectal swab fluid or fecal sample)

^bReal-time PCR-positive; log₁₀ viral titer (GE/ml of rectal swab fluid or fecal sample)

^cEU; euthanized

^dND; not determined

^eVirus shedding of calf # 3 and 4 monitored for longer-term (Figure 3)