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. Author manuscript; available in PMC: 2014 Jan 10.
Published in final edited form as: J Vis Exp. 2012 Aug 6;(66):10.3791/4168. doi: 10.3791/4168

Figure 3. Representative data using the BER THF Molecular Beacon.

Figure 3

AP endonuclease activity specific for hydrolysis of the abasic site analog tetrahydrofuran (THF) was measured in nuclear lysates from the control cell line (LN428) and the MPG over-expression cell line (LN428/MPG). Each lysate was analyzed using either the BER Molecular Beacon Control (LN428, green; LN428/MPG, purple) or the BER THF Molecular Beacon (LN428, blue; LN428/MPG, red). Results are reported as (A) the mean fluorescence response units and (B) the same data normalized to beacon input as described in Section 4 above (THF = tetrahydrofuran).