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. Author manuscript; available in PMC: 2014 Jul 10.

Published in final edited form as: Kidney Int. 2014 Jan 8;86(1):58–66. doi: 10.1038/ki.2013.520

(A) EA.hy926 cells were transfected with three anti-human GAPDH-dsiRNA duplexes (G1, G2 and G3) at indicated concentration for indicated days. Harvested cells were lysed in 1% Triton X-100 lysate buffer and analyzed by Western blot. Upper panel, a representative immunoblot of GAPDH knockdown in cultured EA.hy926 cells. Lower panel, immune blot for β-actin. (B) The number of viable transfected EA.hy926 cells were counted and recorded daily based on trypan blue exclusion. All experiments were repeated for three times with three different batches of cell cultures under similar conditions. Abbreviations include ST, single transfection; DT, double transfection.

(A) EA.hy926 cells were transfected with three anti-human GAPDH-dsiRNA duplexes (G1, G2 and G3) at indicated concentration for indicated days. Harvested cells were lysed in 1% Triton X-100 lysate buffer and analyzed by Western blot. Upper panel, a representative immunoblot of GAPDH knockdown in cultured EA.hy926 cells. Lower panel, immune blot for β-actin. (B) The number of viable transfected EA.hy926 cells were counted and recorded daily based on trypan blue exclusion. All experiments were repeated for three times with three different batches of cell cultures under similar conditions. Abbreviations include ST, single transfection; DT, double transfection.