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. Author manuscript; available in PMC: 2014 Jul 10.
Published in final edited form as: Kidney Int. 2014 Jan 8;86(1):58–66. doi: 10.1038/ki.2013.520

Figure 4. Oxidant stress is characteristic of the Fabry human endothelial cell phenotype.

Figure 4

Figure 4

(A) Comparison of nitrotyrosine content in cultured EA.hy926 cells transfected respectively with GLA-dsiRNA (n = 4), GBA-dsiRNA (n = 4), and GAPDH-dsiRNA (n = 3) as measured by liquid chromatography-electrospray ionization tandem mass spectrometry (LCE-ITMS). The results were normalized to the protein content of tyrosine, the precursor of 3-nitrotyrosine, (B) Levels of other oxidant amino acids; chloro-tyrosine (Chloro-Y), ortho-tyrosine (Ortho-Y), meta-tyrosine (Meta-Y) and dityrosine (Di-Y) in endothelial cells transfected twice with either control-dsiRNA or GLA-dsiRNA as analyzed by LCE-ITMS (n = 3).