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. Author manuscript; available in PMC: 2014 Jul 10.

Published in final edited form as: Kidney Int. 2014 Jan 8;86(1):58–66. doi: 10.1038/ki.2013.520

The detergent soluble proteins were extracted from clarified aortas. Equal amounts of lysate protein (100 μg) were subjected to a 6-13% gradient SDS-PAGE separation. The protein nitration in wild type and Gla null mouse aortas was measured using a monoclonal antibody specific for protein-bound nitrotyrosine. (A) A representative immunoblot image of protein-bound 3NT in mouse aortas. (B) The densitometric values were pooled from three immune blot experiments using total 12 mice (4 groups) at either 2 or 9 months of age. The data represent the mean ± SE (n = 3) for each group.

The detergent soluble proteins were extracted from clarified aortas. Equal amounts of lysate protein (100 μg) were subjected to a 6-13% gradient SDS-PAGE separation. The protein nitration in wild type and Gla null mouse aortas was measured using a monoclonal antibody specific for protein-bound nitrotyrosine. (A) A representative immunoblot image of protein-bound 3NT in mouse aortas. (B) The densitometric values were pooled from three immune blot experiments using total 12 mice (4 groups) at either 2 or 9 months of age. The data represent the mean ± SE (n = 3) for each group.