Fig. 3.

Interferon antagonism activity of MERS-CoV PLpro. HEK293T cells were transfected with plasmids expressing wild type (WT) or catalytic mutant PLpro (CA), plasmids expressing IFNβ-luc (A, B, and C), or NF-κB-luc (D), Renilla-luc, and the stimulator indicated at the top of the figure. For A–C, at 16 h post-transfection, cells were lysed and luciferase activity was measured. For D, at 10 h post-transfection cells were treated with TFNα for 4 h, lysed and luciferase activity was measured. Experiments were performed in triplicate. Error bars represent standards deviation of the mean.