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. Author manuscript; available in PMC: 2014 Jan 27.
Published in final edited form as: Cancer Immunol Immunother. 2007 Dec 4;57(6):897–906. doi: 10.1007/s00262-007-0426-9

Fig. 6.

Fig. 6

Naïve human CTL priming by peptide-pulsed DC. a Naïve human CTL were primed and restimulated with MART-1 peptide-pulsed HLA-A2+ autologous or allogeneic (i.e. matched only for A2) DC. CTL cytotoxicity was measured using a standard 4 h 51Cr release assay against target cells were pulsed with MART-1 or irrelevant peptide. Representative pairs of donors are shown from three paired experiments. Autologous DC priming against MART-1 (filled diamond), against irrelevant target (open triangle); allogeneic DC priming against MART-1 (filled square), against irrelevant target (open square). Total cell culture counts (b) and IFN-γ secretion (c) during naïve priming and restimulation of CTL by autologous (hatched histogram) or allogeneic DC (black histogram) were also measured. A representative pair of donors is shown from three separate paired experiments with similar results