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. Author manuscript; available in PMC: 2015 Apr 23.
Published in final edited form as: Oncogene. 2013 Oct 28;33(43):5100–5108. doi: 10.1038/onc.2013.447

Figure 6. Prohibitin is required for the PMA-dependent differentiation of BASP1 K562 cells.

Figure 6

(A) Vector control V-K562 cells and BASP1-K562 cells were transfected with control siRNA or prohibitin siRNA and 48 hr later were treated with DMSO as a control or treated with 100nM of PMA and then imaged 72hr later. Whole cell-extracts were prepared in parallel and immunoblotted with anti-prohibitin or anti-tubulin antibodies to confirm successful depletion of prohibitin in V-K562 cells and BASP1-K562 cells. (B) As in part A except that quantification of the percentage of arborized cells was performed. Error bars represent SDM of three independent experiments and *p<0.05 by Student's t test. (C) As in part A except that RNA and cDNA were prepared. Expression of P2X5 and MAO were analyzed by qPCR in comparison with GAPDH. Error bars are SDM of three independent experiments (*p<0.05 by Student's t test).