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. Author manuscript; available in PMC: 2015 Feb 19.
Published in final edited form as: Aging Cell. 2014 Feb 4;13(3):478–486. doi: 10.1111/acel.12195

Fig. 4.

Fig. 4

Hematopoietic stem cells (HSCs) in aged Smurf2-deficient mice exhibit enhanced repopulating ability. Donor bone marrow (BM) from wild-type (+/+) or Smurf2T/T (T/T) mice (CD45.2+) mixed at 1:1 ratio with competitor BM of 2-month old wild-type (CD45.1+/CD45.2+) mice are injected into lethally-irradiated recipient mice (CD45.1+). Contribution of donors is determined by CD45 staining. Both young (2-month) and old (24-month) donors were used. (A) Donor (CD45.2+) contributions to B (B220+), T (CD3+) and myeloid (CD11b+) lineages in peripheral blood of recipient mice (N=4) at indicated time post BM transplantation. Two-way ANOVA is used for statistical analysis. *: P<0.05, **: P<0.01, ****: P<0.0001, ns: not significant. (B) Donor contribution to BM and LSK (Lin−Sca1+c-kit++) cells in recipient mice (N=3) at 6 months post BM transplantation. Error bars are SD. Student's t–test is used in statistical analysis.