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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Cancer Immunol Immunother. 2013 Dec 20;63(3):259–271. doi: 10.1007/s00262-013-1506-7

Figure 2. Tumor infiltration by CD8+ T cells, CD4+ T cells and myeloid cells was diminished in mice that lack IFN-α/β signaling.

Figure 2

On Day 13 post-tumor inoculation (Day 6 post-RT) tumors were removed from C57BL/6 or IFNABR KO mice that were either untreated or given 15 Gy RT. Single cell suspensions were obtained using collagenase digestion and cells were incubated with Live/Dead™ reagent, followed by fluorescently-conjugated antibodies. The graphs show absolute numbers of (a) CD8+ CD3+ T cells, (b) CD4+ CD3+ T cells, (c) CD45+ cells, (d) F4/80− CD11c+ CD11b+ DCs and (e) F4/80+ myeloid cells (f) CD3−NK1.1+ cells, from individual tumors calculated after gating out dead cells and pre-gating on CD45+ cells. Data shown are representative of two experiments. (* p<0.05, ** p<0.01, ***p<0.001)