Skip to main content
. Author manuscript; available in PMC: 2014 Aug 11.
Published in final edited form as: Biochemistry. 2014 Jan 6;53(2):333–343. doi: 10.1021/bi4014769

Figure 2.

Figure 2

Specific activity of Av1 measured by the standard assay at pH 7.8 following incubation at pH 9.5, 30 °C for the indicated times. The reactions at pH 9.5 were variations of the full turnover conditions with 0.25 mg Av1. Circles: Av1 and Av2 at CR = 2.5 plus the ATP regenerating system; diamonds: Av1 and Av2 at CR = 2.5 plus the non-hydrolysable ATP analogue AMP-PNP; triangles: Av1 plus ATP regenerating system (minus Av2); and squares: Av1 and Av2 at CR = 2.5 without nucleotide. Error bars indicate standard deviation for duplicate experiments.