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. Author manuscript; available in PMC: 2008 Aug 5.
Published in final edited form as: J Biol Chem. 2008 May 5;283(26):17797–17804. doi: 10.1074/jbc.M800121200

Figure 2. Physical association of αTTP with lipid vesicles.

Figure 2

A) Purified recombinant αTTP (or GST; 1.5 μM) was incubated with the indicated concentration of sonicated vesicles for 30 minutes. Free protein was separated from membrane-bound protein by centrifugal filtration as described in Experimental Procedures. Shown is a representative of three independent experiments. B) Fluorescence emission spectrum of αTTP (0.8 μM; excitation = 295 nm) was monitored in the presence or absence of 15 mM sonicated vesicles. Fluorescence intensities were normalized at the wavelength of maximal emission, and corrected for scattering. Representative of three experiments.