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. Author manuscript; available in PMC: 2014 Jul 1.

Published in final edited form as: Nat Commun. 2014;5:3009. doi: 10.1038/ncomms4009

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a, Wall-eyed stereo view from the periplasmic side of the internal cavity in Mol-A with the N- and C-halves in green and blue, respectively. b, Identical view of Mol-B with N- and C-halves in cyan and blue, respectively. c, Steady-state levels of [³H]melibiose accumulation by intact cells at 10 min presented as histograms. DW2, cells without MelB. Error bar, s.e.m.; n = 2 for all mutants with a single-site mutation, and n = 10 for the WT and DW2. d, Trp→D²G FRET with RSO vesicles. The emission signals were collected at wavelength of 490 nm after excited at wavelength of 290 nm. Λ, adding 10 μM D²G; pink arrows adding 120 mM melibiose; gray arrows adding water; black arrows ↓, adding 20 mM or 50 mM Na⁺ or Li⁺. Black double-headed arrows ↔, Na⁺ or Li⁺ stimulation; pink double-headed arrows , melibiose displacement of D²G.

Inline graphic — a, Wall-eyed stereo view from the periplasmic side of the internal cavity in Mol-A with the N- and C-halves in green and blue, respectively. b, Identical view of Mol-B with N- and C-halves in cyan and blue, respectively. c, Steady-state levels of [³H]melibiose accumulation by intact cells at 10 min presented as histograms. DW2, cells without MelB. Error bar, s.e.m.; n = 2 for all mutants with a single-site mutation, and n = 10 for the WT and DW2. d, Trp→D²G FRET with RSO vesicles. The emission signals were collected at wavelength of 490 nm after excited at wavelength of 290 nm. Λ, adding 10 μM D²G; pink arrows adding 120 mM melibiose; gray arrows adding water; black arrows ↓, adding 20 mM or 50 mM Na⁺ or Li⁺. Black double-headed arrows ↔, Na⁺ or Li⁺ stimulation; pink double-headed arrows , melibiose displacement of D²G.