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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Nat Commun. 2014;5:3009. doi: 10.1038/ncomms4009

Table 2.

Affinity for melibiose and Na+

Melibiose displacement
of D2G
IC50 (mM)		 Na+ activation constant
for D2G FRET
K0.5 (mM)
WT MelBSt 2.65 ± 0.80# 1.92 ± 0.78
R295C 4.04 ± 0.42 3.22 ± 1.07
R141C 5.93 ± 1.61 4.77 ± 0.39
R363C 2.83 ± 0.57 5.51 ± 1.07
#

s.e.m., n = 2.