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. Author manuscript; available in PMC: 2014 Mar 24.
Published in final edited form as: J Neurosci. 2013 May 1;33(18):7890–7904. doi: 10.1523/JNEUROSCI.2787-12.2013

Figure 9.

Figure 9

Autophagosome accumulation in the apical dendrites of vulnerable CSMN. A, Representative image of eGFP+ CSMN in the motor cortex of WT-UeGFP mice at P120. B, Autophagosomes are visualized by LC3B expression. C, Representative image of eGFP+ CSMN in the motor cortex of hSOD1G93A-UeGFP mice at P120. The vacuolated apical dendrites of vulnerable CSMN are observed. D, Anti-LC3B immunocytochemistry reveal the presence of autophagosomes, especially along the apical dendrites of eGFP+ CSMN in the motor cortex of hSOD1G93A-UeGFP mice. E, Bar graph representation of average percentage CSMN containing autophagosomes along apical dendrites in WT-eGFP and hSOD1G93A-UeGFP mice at P30, P60, P90, and P120. One-way ANOVA followed by Tukey’s post hoc multiple-comparison test and Student’s t test: #p < 0.05; *p < 0.05; ***p < 0.0001. Error bars indicate SEM. Scale bar, 20 μm.