Table 3.

In vivo experimental parameters in the study by Konishi et al. [43] and their adaption in the present study for in silico simulations using the Cochlear Fluids Simulator (V 3.080).

	Konishi et al. (1984)	Cochlear Fluids Simulator (V 1.6i, modified)
species	guinea pig	guinea pig
tracer substance (TS)	THO	THO (MW: 22.0315)
TS – diff.-coeff.		2.3004 × 10−5 cm2·s−1
TS – conc. in perfusate	2 μCi ml−1 (normalised to 100%)	100%
TS – perfusion rate	8 μl·min−1	8 μl·min−1
TS – perfusion period	3 – 20 min	0 – 120 min
TS – entry site	basal turn	basal turn (0.1 mm from base)
TS – exit site	helicotrema	apical end of SV (15.5 mm from base) and ST (16.2 mm from base)
TS – method of conc. measurements	endolymph sampling	continuous measurements
location of measurement	SM (basal turn)	SM, SV, ST (1 mm from base)
scala-scala communications*	–	SV-SM = 2.2 min ST-SV = 9999 min ST-SM = 4.6 min
scala-blood communications*	–	SV-blood = 1.6 min ST-blood = 1.6 min SM-blood = 15 min

Software settings and parameters that are not explicitly mentioned were applied in the standard configuration.

^*, software parameters defined on the basis of experimental data from [43].