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. Author manuscript; available in PMC: 2015 Jan 21.
Published in final edited form as: J Phys Chem A. 2014 Jan 31;118(6):993–1003. doi: 10.1021/jp4107475

Figure 6.

Figure 6

Images of iron-loaded Sepia eumelanin at various total power levels taken in succession with 720 nm pump, 815 nm probe. Color scheme: Green pixels have a pump–probe response similar to that of the iron-loaded eumelanin standard. Red pixels are similar to the response of EDTA-washed eumelanin. The black and white overlaid image represents the multiphoton fluorescence channel. The white box (with zoom inset) shows a melanin granule that first pump–probe photobleaches and then begins to fluoresce. The magenta arrow shows a granule of melanin where the pump–probe response form gradually shifts from similar to iron-loaded eumelanin to resembling that of EDTA-washed eumelanin.