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. Author manuscript; available in PMC: 2014 Oct 17.
Published in final edited form as: J Vis Exp. 2013 Oct 17;(80):e50638. doi: 10.3791/50638

Figure 2. Caco-2 monolayers as a model for transepithelial transport.

Figure 2

Caco-2 cells were grown on transwell inserts at 1.5×105 cells/cm2. (A) Transepithelial electrical resistance (TEER) was measured to assess monolayer integrity. Data are shown as means ± S.E.M; n=3. (B) Fluorescence microscopy of tight junctions immunolabeled with anti-occludin and Texas Red-labeled secondary antibody, on Days 5 or 14 (low versus high TEER values, respectively). Reproduced from Ghaffarian et al., J Controlled Rel, 2012, 163(1):25–33.