Table 2.
Gene Set Enrichment Analysis for KEGG Pathways Overrepresented in Transcripts Affected by Sample Preservation
| KEGG Pathway | p-Value | Adjusted p-Value* | Number of Genes |
|---|---|---|---|
| Transcripts higher in RNAlater | |||
| Focal adhesion | 2.53E-08 | 4.28E-06 | 184 |
| ECM-receptor interaction | 1.14E-05 | 9.62E-04 | 78 |
| B cell receptor signaling pathway | 6.41E-05 | 3.61E-03 | 59 |
| TGF-beta signaling pathway | 1.90E-04 | 8.03E-03 | 76 |
| Citrate cycle (TCA cycle) | 1.16E-03 | 3.80E-02 | 27 |
| Chondroitin sulfate biosynthesis | 1.59E-03 | 3.80E-02 | 15 |
| Colorectal cancer | 1.99E-03 | 3.80E-02 | 79 |
| Pyruvate metabolism | 2.01E-03 | 3.80E-02 | 33 |
| Proteasome | 2.03E-03 | 3.80E-02 | 41 |
| Cell cycle | 2.52E-03 | 4.26E-02 | 101 |
| p53 signaling pathway | 5.72E-03 | 8.55E-02 | 58 |
| Transcripts Higher in Snap Frozen | |||
| Neuroactive ligand-receptor interaction | 1.70E-20 | 2.88E-18 | 180 |
| Olfactory transduction | 9.79E-15 | 8.27E-13 | 54 |
| Retinol metabolism | 2.53E-07 | 1.42E-05 | 34 |
| Ribosome | 4.65E-07 | 1.83E-05 | 65 |
| Drug metabolism - cytochrome P450 | 5.40E-07 | 1.83E-05 | 45 |
| Taste transduction | 7.43E-07 | 2.09E-05 | 29 |
| Linoleic acid metabolism | 9.11E-05 | 2.20E-03 | 22 |
| Metabolism of xenobiotics by cytochrome P450 | 1.19E-04 | 2.52E-03 | 40 |
| Maturity onset diabetes of the young | 6.87E-04 | 1.29E-02 | 11 |
| Cytokine-cytokine receptor interaction | 1.71E-03 | 2.89E-02 | 190 |
| Nitrogen metabolism | 3.59E-03 | 5.51E-02 | 22 |
| Autoimmune thyroid disease | 4.60E-03 | 6.46E-02 | 38 |
| Calcium signaling pathway | 4.97E-03 | 6.46E-02 | 141 |
*
Adjusted p-value by the Benjamini Hochberg approach to account for multiple testing (Benjamini and Hochberg, 1995)