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. Author manuscript; available in PMC: 2014 Dec 19.
Published in final edited form as: Insect Mol Biol. 2014 Apr 29;23(4):457–465. doi: 10.1111/imb.12094

Figure 4. Defensin-2 associates with R. montanensis in vivo.

Figure 4

(A) R. montanensis-infected tick midgut incubated with immune sera. (B) R. montanensis-infected tick midgut incubated with control sera. R. montanensis-infected D. variabilis midguts 48 hours post-infection were probed with rabbit defensin and mouse R. montanensis antisera or rabbit and mouse preimmune serum. Rabbit and mouse antibodies were detected with anti-rabbit Alexa-488 (defensin) or anti-mouse Alexa-594 (rickettsia), respectively. SYTOX Blue was used to visualize the nucleus. DIC, differential interference contrast. Bars represent 5 μm.