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. Author manuscript; available in PMC: 2015 Feb 12.
Published in final edited form as: J Am Chem Soc. 2014 Feb 25;136(9):3640–3646. doi: 10.1021/ja4130302

Figure 2.

Figure 2

(A) Enzymatic consumption of M-IV (calculated from HPLC areas under curve, AUC) fit into the one phase exponential decay curve. The rate constants (k) are shown in Table 1. (B) The effects of catalase (20 U), superoxide dismutase (2 U), ascorbate (10 mM), and the combination of these three ROS scavengers on the activity of MycG/RhFRED against M-IV. Closed bar, the ratio of demethylated product/oxidized products relative to that of the control reaction without addition of any scavengers; open bar, the overall conversion percentage calculated based on the substrate M-IV consumption. The percentage numbers from the control reaction are arbitrarily assigned to be 100%.