Fig. 2.

Localization of H2A variants by immunofluorescence. a mH2A.1 localizes to the Xi, note also staining throughout the nucleus. b mH2A.1 localizes to SAHFs in cells induced to senesce by oncogenic RAS signaling, note also the presence of Xi. c Phosphorylated mH2A.1 on S137 (S137ph) is excluded from the Xi and has a distinct nuclear localization pattern compared to mH2A.1. d mH2A.2, like mH2A.1, localizes to the Xi and throughout the nucleus. e y-H2A.X (S139ph) localizes to IRIF upon DNA damage induced by γ-irradiation. All imaging performed in IMR-90 female primary lung fibroblasts at a 40X magnification. Histones or histone modification antibodies were labeled with Alexa Fluor 488 (green) and DAPI (blue) was used to visualize the nucleus