REAGENTS:

Name	Company or ingredients	Catalog Number	Comments
Antimycin A	Sigma-Aldrich (St. Louis, MO)	1397-94-0	Dissolved in ethanol to a 2 mg/mL stock solution.
SGIyc (synthetic glycerol-based) yeast growth medium	0.67% Yeast nitrogen base without amino acids	N/A	Dissolve in H2O. Adjust pH to 5.5 with NaHCO3. Autoclave.
	3% Glycerol
*omit for SGIyc-Ura	0.05% Glucose
**omit for SGIyc-Leu	2 mg/mL adenine
	2 mg/mL uracil*
	1 mg/mL L-arginine
	1 mg/mL L-histidine
	1 mg/mL L-leucine**
	3 mg/mL L-lysine
	2 mg/mL L-methionine
	4 mg/mL L-phenylalanine
	2 mg/mL L-tryptophan
	3 mg/mL L-tyrosine
SC (synthetic complete, glucose-based) yeast growth medium	0.67% Yeast nitrogen base without amino acids	N/A	Dissolve in H2O. Adjust pH to 5.5 with NaHCO3. Autoclave.
	3% Glucose
*omit for SGIyc-Ura	2 mg/mL adenine
**omit for SGIyc-Leu	2 mg/mL uracil*
	1 mg/mL L-arginine
	1 mg/mL L-histidine
	1 mg/mL L-leucine**
	3 mg/mL L-lysine
	2 mg/mL L-methionine
	4 mg/mL L-phenylalanine
	2 mg/mL L-tryptophan
	3 mg/mL L-tyrosine
Valap	Vaseline petroleum jelly, hard paraffin, lanolin	N/A	Combine ingredients in a 1:1:1 (w:w:w) ratio. Melt by submerging in a 70°C H2O bath. Aliquot into glass petri dishes. Store at room temperature.