. Author manuscript; available in PMC: 2015 Mar 14.

Published in final edited form as: J Am Chem Soc. 2014 Mar 21;136(13):4901–4904. doi: 10.1021/ja5016052

Table 1.

TTNs and K_d values of unnatural substrates with PikC_D50N and PikC_D50NH238A mutants.

substrate	TTN (D50N)	TTN (D50NH238A)	K_d (D50N) μM	K_d (D50NH238A) μM
1	896 ± 0.71	842 ± 16	19	ND
8a	260 ± 11	572 ± 18	118 ± 21	11 ± 0.56
8b	544 ± 23	870 ± 38	81 ± 15	161 ± 25
10	452 ± 5.0	853 ± 38	123 ± 14	168 ± 19
12a	456 ± 5.0	542 ± 30	47 ± 3.0	19 ± 1.3
12b	485 ± 21	538 ± 20	28 ± 5.4	21 ± 0.70
14	152 ± 11	491 ± 26	ND	ND
16a	602 ± 3.5	816 ± 33	ND	ND
16b	580 ± 11	922 ± 35	ND	ND

The measurement of K_d values for the benzylic amine substrates was inhibited by the background absorbance of these compounds, thus, binding constants were not obtained for 14, 16a, 16b.