Table 2. No significant difference in proportion of phagocytically active hemocytes at one and five weeks of age.

He-Gal4, UAS-GFP flies injected with fluorescent E. coli assayed ninety minutes after infection. If GFP-positive cells had at least one phagocytic event, they were counted as active; GFP-positive cells with no events were classified as inactive. Only GFP-positive cells associated with the dorsal vessel were scored. We analyzed the data using a maximum likelihood analysis with nested logistic regression and a binomial distribution, and detected no significant difference in active proportion with age: Low titer (1:50^th): χ²= 0.001, p=0.97; Regular titer: χ²=1.13, p=0.28. Ages given are either one week post eclosion (4–7 days) or 5 weeks post eclosion (35–39 days).

Titer	Age	Number of flies and (number of GFP+ cells) analyzed	% Active	% Inactive
Low	1 Week	n=5 (153 cells)	61	39
Low	5 Weeks	n=4 (103 cells)	64	36
Regular	1 Week	n=16 (253 cells)	72	28
Regular	5 Weeks	n=11 (245 cells)	85	15