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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Cancer Sci. 2009 Nov 6;101(3):707–712. doi: 10.1111/j.1349-7006.2009.01425.x

Fig. 3.

Fig. 3

Inhibitor of DNA binding 2 (ID2) overexpression rescues Du145 cells from apoptosis. (A) Semiquantitation of ID2 expression by RT-PCR on RNA from Du145 cells transfected with pCMVscript (lanes 1 and 2) or pCMV-ID2 (lanes 3 and 4). cDNA templates (200 ng) from Du145 transiently transfected with pCMV script or pCMV-ID2 were titrated to the indicated dilutions. PCR were carried out using primers specific for ID2 or β-actin. The PCR products were then electrophoresed and visualized in 2% agarose gel. (B) Representative samples of FACS analysis of Du145 transfected with pCMV-ID2 or pCMVscript, and treated with or without (−)-epigallocatechin-3-gallate (EGCG). (C) Means of DU145 cell deaths by FACS of (B). Each condition was triplicated in the analysis. Standard deviations are indicated.