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. Author manuscript; available in PMC: 2014 Aug 7.
Published in final edited form as: Chem Biol. 2014 Apr 24;21(5):689–699. doi: 10.1016/j.chembiol.2014.03.009

Figure 4. Effects of inositol phosphate analogues upon PPIP5K2KD activities: ATPase, 1,5-[PP]2-InsP4 dephosphorylation and InsP6 phosphorylation.

Figure 4

Dose-response curves are shown for the indicated inositol phosphate analogues and their stimulation of ATPase activities (Panel A), and their inhibition of ADP-driven dephosphorylation of 100 nM [PP]2-InsP4 (Panel B). Error bars represent standard errors (n=3). The EC50 and IC50 values for the molecules tested are given in the key to the right of panels A and B. Panel C shows a Dixon plot of the inhibition of InsP6 kinase activity of PPIP5K2KD by 2,5-di-O-Bn-InsP4 (10); the data are from a representative experiment (one of three), performed in duplicate. Insets to each panel illustrate the reaction being assayed.