Ang1-256 Monomer Reduces Cardiac Hypertrophy In Vivo. Ang1-256/pcDNA (ang1-256) or pcDNA (control) were retroorbitally injected into C57 mice. On day 2, mice were given PE or saline via pumps (n=4/group, studies done in duplicate). On day 6, (A) PE increased LV/tibia ratios (*p=0.009) and ang1-256 monomer blunted PE-induced increases in LV/tibia ratios (**p=0.02). PE upregulated mRNA levels of (B) ANP, (C) BNP, (D) collagen III, (E) skeletal actin and (F) βMHC (**p≤0.02), but not (G) SMA (real-time PCR). Ang1-256 monomer reduced ANP, BNP, collagen III, skeletal actin, βMHC, and SMA mRNA levels in the absence (*p≤0.03; ****p=0.006) or presence of PE (***p≤0.03).