Ang1-256 Monomer Does Not Change LV Tie2 Phosphorylation, but Increased ILK. (A–C) Ang1-256/pcDNA (ang1-256) or pcDNA (control) were retroorbitally injected into C57 mice. Using tie2 immunoprecipitation and Western blotting for pTyr and tie2 (studies done in duplicate), we found that tie2 phosphorylations were unchanged in adult heart on (A) days 1 and 2 post plasmid injection and (B) day 6 in PE-induced cardiac hypertrophy. (C) Six days after plasmid injection, Western blotting of mouse LV shows that ILK protein levels increased by ang1-256 either alone or with PE-induced cardiac hypertrophy (n=4/group, studies done in duplicate, *p=0.01, **p=0.03). ILK was normalized to GAPDH.