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. Author manuscript; available in PMC: 2014 Aug 21.
Published in final edited form as: J Mol Med (Berl). 2014 May 3;92(9):951–959. doi: 10.1007/s00109-014-1157-y

Figure 1. Sn binds Group B Streptococcus (GBS) and is upregulated by inflammatory stimuli.

Figure 1

(A) FITC-labeled GBS were added to plates coated with human Sn (hSn), murine Sn (mSn) or control human IgG (hIgG). Binding of bacteria to applied proteins was measured using a fluorescent plate reader after extensive washes. Data shown are means ± sem and the GBS binding difference between control and Sn was determined by two-way ANOVA with Bonferroni posttest. ***P < 0.001; **P < 0.01; *P < 0.05. Experiments have been done with three biological replicates and repeated twice. (B) MBDMs were stimulated with various bacterial components or IFN-α for 2 d, stained with anti-Sn mAb and subjected to FACS analysis to examine Sn expression. Black thin line: isotype control; gray thick line: anti-Sn mAb. Representative data from one of the two reproducible independent experiments was shown.