. Author manuscript; available in PMC: 2016 Mar 1.

Published in final edited form as: Brain Pathol. 2014 Aug 19;25(2):171–181. doi: 10.1111/bpa.12165

Table 1.

Radioligands used to assess nAChRs and 5-HT markers in the fetal baboon occipital lobe of the cerebral hemisphere

Ligand	Concentration	Displacer	Imaging	Detects
³H-Epibatidine	0.5nM (56 Ci/mmol); 60min at RT	300μM nicotine (Sigma-Aldrich, St. Louis, MO)	BAS system – 3 wks	heteromeric α2- 4, β2, β4 nAChRs
¹²⁵I-Bungarotoxin	1.5nM (120 Ci/mmol); 2hrs at 4°C	1mM nicotine (Sigma-Aldrich, St. Louis, MO)	¹²⁵I film - 7 days	homomeric α7 nAChRs
³H-8-OH-DPAT	4nM (120 Ci/mmol); 1hr at RT	10μM serotonin (Sigma-Aldrich, St. Louis, MO)	BAS system – 4 wks	5-HT_1A autoreceptor
¹²⁵I-DOI	86.3pM (2200 Ci/mmol); 60min at RT	10⁻⁶M ritanserin (Sigma-Aldrich, St. Louis, MO)	¹²⁵I film - 7 days	5-HT_2A receptor
¹²⁵I-RTI-55	0.15nM (2200 Ci/mmol); 90min at RT	100nM citalopram (Sigma-Aldrich, St. Louis, MO)	¹²⁵I film – 9 hours	5-HT transporter, 5- HTT