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. Author manuscript; available in PMC: 2014 Dec 3.
Published in final edited form as: Pharmacol Res Perspect. 2014 Jul 23;2(5):e00061. doi: 10.1002/prp2.61

Figure 3. Exacerbation of CCl4-induced p53 acetylation and PUMA expression by ethanol were attenuated in livers of ΔHepHIF1α−/− mice.

Figure 3

C57BL/6 wild-type (WT) and ΔHepHIF1α−/− mice were allowed were allowed free access to diets with ethanol (11% of kcal) or pair-fed controls for 4 days followed by CCl4 injections, as described earlier. (A) Ac-p53/K386, (B) Ac-p53/K386 (green) and collagen 1 (red) co-staining or (C) PUMA. Images were acquired using a 40X objective. PUMA (green) was co-stained with the nuclear stain DAPI (blue). (D) Total number of PUMA+ cells was counted. Values with different alphabetical superscripts were significantly different from each other, p< 0.05. n=4–6.