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. Author manuscript; available in PMC: 2014 Dec 3.
Published in final edited form as: Pharmacol Res Perspect. 2014 Jul 23;2(5):e00061. doi: 10.1002/prp2.61

Figure 4. Ethanol-induced exacerbation of CCl4–induced Sirius red staining was reduced in livers of ΔHepHIF1α−/− mice.

Figure 4

C57BL/6, HIF1αfl/fl, Alb-cre and ΔHepHIF1α−/− mice were allowed free access to diets with ethanol (11% of kcal) or pair-fed controls for 4 days followed by CCl4 injections, as described in methods. (A) Paraffin-embedded liver sections were de-paraffinized and stained for Sirius red staining. Images are acquired using 20X objective. (B) Areas positive for Sirius red were quantified using Image Pro-Plus software and analyzed. Values with different alphabetical superscripts were significantly different from each other, p< 0.05. n=4–6.