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. Author manuscript; available in PMC: 2015 Aug 30.
Published in final edited form as: Virus Res. 2014 Jun 5;0:177–188. doi: 10.1016/j.virusres.2014.05.023

Fig. 4.

Fig. 4

The effect of alanine residue substitution in positions 581–586 on the cleavage properties of spike protein and viral recovery. Immunoprecipitation of 293T cells transfected with the expression vector pCAGGS-S or the expression vector encoding the alanine substituted residues in positions 581–586 of the spike protein (pCAGGS-S (581–586)). The cells were metabolically labeled with [35S]-methionine and cysteine for eight hours at 37°C and the lysate was precipitated with the monoclonal anti-S antibodies A2.1 and A2.3 S uncleaved denotes the 180 kDa uncleaved spike protein, and S cleaved denotes the 90kDa cleaved S1 and S2 subunits.