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. Author manuscript; available in PMC: 2015 Jun 17.
Published in final edited form as: Mol Pharm. 2014 Jun 25;11(11):4007–4014. doi: 10.1021/mp500306k

Scheme 1. Schematic Illustration of the Synthesis of 64Cu-MSN-800CW-TRC105(Fab)a.

Scheme 1

aUniform MSN nanoparticle (1) was first modified with –NH2 groups with APS to form MSN-NH2 (2). As-synthesized MSN-NH2 was then subjected to 800CW and NOTA conjugation to yield NOTA-MSN-800CW-NH2 (3). Afterwards, PEGylation step was introduced to render the stability of NOTA-MSN-800CW-PEG-Mal (4) in PBS, at the same time adding maleimide groups. NOTA-MSN-800CW-PEG-TRC105(Fab) (5) could be obtained by reacting TRC105(Fab)-SH with 4 at room temperature. 64Cu-labeling was performed in the last step to generate 64Cu-NOTA-MSN-800CW-PEG-TRC105(Fab) (6), short for 64Cu-MSN-800CW-TRC105(Fab).