Skip to main content
. Author manuscript; available in PMC: 2014 Aug 14.
Published in final edited form as: J Vis Exp. 2014 Jun 17;(88):10.3791/51425. doi: 10.3791/51425

Figure 4. Regulated degradation of β-catenin-luciferase in Xenopus extract can be adapted to a high-throughput format.

Figure 4

A representative checkerboard analysis for β-catenin-luciferase using MG132 (450 μM) as an inhibitor of β-catenin degradation. Shaded columns indicate MG132-treated wells, and lighter columns represent vehicle (DMSO)-treated wells. Quantification and Z-factor calculation result in a score of 0.3 indicating an acceptable assay for potential use in high-throughput screening.