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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Mol Oncol. 2014 Jul 29;9(1):44–57. doi: 10.1016/j.molonc.2014.07.013

Figure 3. Co-culture of T cells promoted PCa invasion.

Figure 3

A.CMs collected from PCa/T cells co-culture could promote PCa invasiveness using in vitro matrigel invasion assay. The PCa/T cells were co-cultured in 0.4 μM transwell plate for 2 days. The CM or control media were collected, diluted 1:1 with 10% FBS RPMI media, and placed into the lower chamber of transwells. The 1×105 PCa cells (without any pre-treatment) were plated onto upper insert chamber pre-coated with matrigel for 36 hr invasion assay. B. Toluidine blue staining results showed the PCa/T cells co-cultured CM can promote PCa cell invasion. Invasion assay has been performed on 3 different PCa cell lines, LNCaP, C4-2 and CWR22RV1(22RV1) and 2 different T cells, Molt-3 and HH. (*P<0.05).C. 3D invasion assay showed that more acini-like structures formed when treating the parental CWR22RV1 cells with CWR22RV1/T cell co-culture CM (200×) (*P<0.05).