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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Mol Oncol. 2014 Jul 29;9(1):44–57. doi: 10.1016/j.molonc.2014.07.013

Figure 4. T cells can down-regulate AR expression and up-regulate MMP9 expression of PCa cells after co-culture.

Figure 4

A. Q-PCR detection showed AR mRNA was down-regulatedin PCa cells, LNCaP, C4-2 and CWR22RV1 (22RV1), after co-culture with HH or Molt-3 cells for 2 days (*P<0.05). B. Western Blot results showed AR expression of LNCaP, C4-2 and 22RV1 cells decreased after co-culture with HH or Molt-3 cells for 2 days. C. T cell co-culture could not effectively change invasion in PCa cells with AR knockdown by siRNA. We used lentiviral AR-siRNA to knock down AR expression in PCa. T cells co-culture failed to promote invasion of PCa cell with AR-siRNA compared with PCa cell with control si-Luc(*P<0.05). D. Four metastasis-related genes were detected by q-PCR. TGF-beta1, MMP9 and ZEB1 mRNA level were up-regulated in PCa cells after co-culture with T cells, however the MMP9-inhibitor gene, TIMP1 decreased in PCa cells(*P<0.05). E. MMP9 expressions of LNCaP, C4-2 and 22RV1 cells are up-regulated after co-culture with HH or Molt-3 cells using Western Blot assay(*P<0.05). F. Blocking MMP9 by MMP9 inhibitor can partly reverse T cell-promoted PCa invasion(*P<0.05).