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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Virology. 2014 Aug 9;0:287–295. doi: 10.1016/j.virol.2014.07.025

Figure 3. The effects of cathepsins on the cleavage of PEC VP1 and VP2.

Figure 3

Concentrated PEC were incubated with a recombinant cathepsin L or cathepsin B in a cathepsin reaction buffer at pH 4.0 or pH 5.5, respectively, for 2 h at 37°C. Mock-treatment samples were incubated in PBS at pH 7.0. The samples were then subjected to SDS-PAGE, transferred to nitrocellulose membrane, probed with antibodies against PEC VP1 or VP2 and visualized by chemiluminescence. Uncleaved or cleaved VP1 and VP2 are indicated by arrows.