(a) Constitutive expression of IL-33 in MS, HC and OND patients in CD3+, CD14+ and CD19+ cells determined by intracellular cytokine staining and flow cytometry. Results are expressed as percent positive cells. Mean values are shown as horizontal bars, p<0.05 between MS and controls, for all subsets examined.
(b) As in (a) except PBMC were stimulated with LPS followed by intracellular cytokine staining to determine induced IL-33 expression in MS, HC and OND in CD14+ and CD19+ subsets of PBMC. Mean value are shown as horizontal bars, p<0.05 between MS and controls, for all subsets examined.
* The samples for these experiments were obtained from MS clinic at Vanderbilt.