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. Author manuscript; available in PMC: 2014 Dec 10.
Published in final edited form as: Bone Res. 2014;2:14011. doi: 10.1038/boneres.2014.11

Figure 1. Effects of over-expressed human hnRNPC1 and C2 on vitamin D receptor (VDR)-mediated gene transcription in human and mouse osteoblast-like cells.

Figure 1

Effect of over-expression of human hnRNPC1 (C1) and/or human hnRNPC2 (C2) on 1,25(OH)2D-induction of the vitamin D-target genes CYP24A1/Cyp24a1 and DDIT4/Ddit4 in A) mouse MC3T3 osteoblastic cells and B)human MG-63 osteoblastic cells. Cells were treated with or without 10 nM 1,25(OH)2D for 6 hrs, 24 hrs post-transfection with either empty vector (C), C1, C2 or C1 and C2 (C1/C2). Data show mean±SD (n=3 separate cultures) fold-change in mRNA expression for CYP24A1/Cyp24a1 and DDIT4/Ddit4 for each transfected cell type following treatment with 1,25(OH)2D relative to vehicle-treated control cells. ***statistically different from empty vector controls (C), p < 0.001, **statistically different from C, p < 0.01.