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. Author manuscript; available in PMC: 2014 Dec 10.
Published in final edited form as: Bone Res. 2014;2:14011. doi: 10.1038/boneres.2014.11

Figure 5. Cell-type specific action and temporal regulation of the P2A hnRNPC1/C2 construct.

Figure 5

A) Endogenous mouse (mu) type I collagen expression was monitored over a 96 hr period under the different transient transfection conditions. For both transfected constructs into MC3T3-E1 cells, col1a1 mRNA expression was delayed when compared to the untransfected controls. In J774A.1 mouse monocytes the expression of endogenous mu type I collagen was variable between the transfected P2A construct and untransfected control, and unregulated in the empty vector control. B) Human (hu) hnRNPC mRNA levels were monitored in the mouse col1a1-promoter driven P2A construct transfected cells at 96 hrs post transfection. Hu hnRNPC mRNA was temporally regulated in a similar fashion as mu type I collagen in MC3T3-E1 osteoblasts. No temporal regulation of hu hnRNPC mRNA was observed in J774A.1 mu monocytes transfected with the P2A construct, suggesting the mu col1a1 2.3kB promoter was bone-specific. C) After 96 hrs of transfection, MC3T3-E1 cells harboring the P2A hnRNPC1/C2 construct express hu hnRNPC with mRNA levels approximately 125-fold higher relative to the matching empty vector control when compared to the J774A.1 mouse monocytic cell line.