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. Author manuscript; available in PMC: 2015 Jan 28.
Published in final edited form as: J Neurochem. 2014 Sep 19;132(1):85–98. doi: 10.1111/jnc.12934

Figure 2. E-FABP is up-regulated under PAM-induced lipotoxicity (PAM-LTx) in NGFDPC12 cells.

Figure 2

(A) Cells were treated with control medium (CTL) or PAM at the concentration of 75 μM, 150 μM or 300 μM for 6 to 48 hrs. Quantification of E-FABP mRNA levels was performed by real-time RT-PCR. (B) Cells were treated with CTL or 300 μM PAM for 6 to 24 hrs. Quantification of E-FABP protein levels was done by Western blots followed by densitometry analysis, using β-actin as the loading control. (C) A representative Western blot for E-FABP (15kDa) and β-actin (42kDa) of NGFDPC12 cells treated with 300 μM or 150 μM PAM for 6 to 24 hrs. The data represent mean ± SEM of three independent experiments. Significance symbols: **=p<0.01 and ***=p<0.001 (compared to control group).