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. Author manuscript; available in PMC: 2015 Jan 28.
Published in final edited form as: J Neurochem. 2014 Sep 19;132(1):85–98. doi: 10.1111/jnc.12934

Figure 6. Lowering E-FABP levels in NGFDPC12 cells by siRNA increases ROS accumulation and PAM-LTx-induced cell death.

Figure 6

(A) Left: Three-day-differentiated PC12 cells were transfected with siControl or siE-FABP for 5 days. A representative E-FABP Western blot is shown. Right: siControl and siE-FABP transfected cells were treated with control medium or 300 μM PAM for 12 and 18 hrs. Relative E-FABP mRNA levels were determined by real-time RT-PCR. (B) Cellular ROS levels of transfection reagent (vehicle), siControl and siE-FABP treated cells were measured by H2DCFDA method at 12 and 18 hrs after PAM (or control medium) treatment. (C) Cell viability of siControl and siE-FABP cells were measured by WST-1 assay at 24 and 48 hrs after PAM treatment. CTL: control medium at 48 hrs. The data represent mean ± SEM of three independent experiments. Significance symbols: *=p < 0.05, **=p<0.01 and ***=p<0.001. Significance symbols are shown above bars when compared to control group and are shown above lines when compared between two linked groups.