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ELL2 influences cyclin B2 and UPR expression by stimulating transcription/ RNA yields from their promoters.
a. The mouse cyclin B2 promoter ( -1188 to 0) was cloned into pGL4.10, the luciferase reporter plasmid. 293T cells were co-transfected with the CycB2 reporter and the indicated cDNA plasmids and luciferase activity measures with a luminometer.
b. The ELL2 promoter (-3000 to 0) was cloned into pGL4.11 luciferase reporter and transfected with the indicated cDNA plasmids.
