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. Author manuscript; available in PMC: 2014 Oct 7.
Published in final edited form as: Mol Pharm. 2014 Sep 2;11(10):3737–3746. doi: 10.1021/mp500497p

Figure 1.

Figure 1

Genotyping results for the identification of humanized PEPT1 mice. Genomic DNA was extracted from mouse tail biopsies and genotyped by PCR using specific primers, as described previously.23 The DNA ladder, consisting of 100 bp repeats, was used to determine the size of PCR products. mPept1−/−/hPEPT1+/− represents the positive screen for humanized PEPT1 (huPEPT1) mice, mPept1−/−/hPEPT1−/− the negative screen for humanized PEPT1 mice, mPept1+/+ the wildtype mice, mPept1−/− the Pept1 knockout (KO) mice, and RP11–782G13 the purified BAC DNA (used to inject fertilized eggs in generating huPEPT1), which serves as a positive control.