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. Author manuscript; available in PMC: 2015 Sep 22.
Published in final edited form as: Bioconjug Chem. 2014 Oct 3;25(10):1801–1810. doi: 10.1021/bc500312w

Figure 4.

Figure 4

SDS-PAGE of (A) SE-HPLC-isolated ICG-sOSu-panitumumab conjugates and aggregates, and (B) reaction mixture, under non-reducing and reducing conditions with β-mercaptoethanol. Panitumumab served as a reference. Colloidal blue protein staining and optical imaging were performed. M: marker; 1: Intact panitumumab antibody; 2: 1; 3: 2; 4: 3; 5: ICG-sOSu aggregates (from 20× reaction, peak with RT=12.5 min.); 6: ICG-sOSu aggregates (from 20× reaction, peak with RT=14.5 min.); 7: ICG-sOSu-panitumumab reaction mixture (10×, without purification); 8: ICG-sOSu alone incubated in conjugation buffer for 1 h at 37 °C as a control. White arrow: heavy chain; yellow arrow: light chain; blue arrow: free dye.