. Author manuscript; available in PMC: 2014 Dec 5.

Published in final edited form as: Nat Commun. 2014 Nov 18;5:5482. doi: 10.1038/ncomms6482

Table 1.

Stable MDCK cells with tetracycline-inducible expression of mouse PC1 proteins

MDCK cells	PC1 variant expressed	Description
MDCK^PC1WT	PC1^WT	Full-length wild-type PC1 with a C-terminal FLAG tag
MDCK^PC1–2M	PC1^2M	Full-length PC1 with a C-terminal FLAG tag, containing amino acid substitutions at L4219P and A4222P within the coiled-coil domain
MDCK^PC1-R/X	PC1^R/X	PC1 terminating at R4218X with a C-terminal FLAG tag before the stop codon
MDCK^PC1V	PC1^V	Full-length PC1 with a C-terminal FLAG tag, containing amino acid substitution T3041V at GPS cleavage site